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rbgA [2018-02-12T14:57:33.000Z]
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rbgA [2018-02-12T14:57:33.000Z]

assembly of the 50S subunit of the ribosome
locus
BSU16050
pI
9.53
mw
31.83 kDa
protein length
282 aa Sequence Blast
gene length
846 bp Sequence Blast
function
ribosome assembly
product
GTPase
essential
yes
synonyms
ylqF
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.6|Ribosome assembly]
  • [category|SW 3|Information processing] → [category|SW 3.5|Targets of second messengers] → [category|SW 3.5.3|Targets of (p)ppGpp]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • [category|SW 6|Groups of genes] → [category|SW 6.3|GTP-binding proteins]

    • Gene

    Coordinates
    1,676,532 → 1,677,380

    Phenotypes of a mutant

  • essential [Pubmed|12682299]
  • depletion confers a slow growth phenotype, and induces an abnormality of the ''in vivo'' ribosome profile, with an increased level of 45S precursors and a decreased level of 70S ribosomes [Pubmed|24335279,23700310]
  • premature 45S particles accumulated in RbgA-depleted cells lack several proteins, including [gene|C5CAED1256F082E722EBA0A72647AA043B5681DB|L16]]], [gene|4626C659D6F1A81CCC94030F1CBAF7FEC3DE19CA|L27]]], [gene|A58851E0B5C4DE4D6F3C16FF8C009001E393C3B8|L28]]], [gene|D73FC9CAD929919050837824B4794F9FC9E83052|L33]]], [gene|7F22743ED669DBCD4089EA2F86B8FFD924F8C2B9|L35]]] and [gene|F69B475C014087C6DEBE2F50F91862A623D21D1E|L36]]] [Pubmed|24335279,23700310]

    • The protein

    Catalyzed reaction/ biological activity

  • proper positioning of [gene|20485A785D3AF82889F2018D3EA5B5FA33E64E95|L6]]] in the large subunit of the [SW|ribosome] prior to incorporation of [gene|C5CAED1256F082E722EBA0A72647AA043B5681DB|L16]]] [Pubmed|25330043]
  • Binds and hydrolyzes GTP and readily exchanges GDP for GTP

    • Protein family

  • MTG1 subfamily (according to Swiss-Prot) Era/Obg family

    • Paralogous protein(s)

  • [protein|CB68B3393CA7862C45C624AF7B1ADD188243A01F|Era], [protein|84E7D672DB40B114DC4D96A4D8834958B47401A7|Obg], [protein|B2270316642C66BC2DA86EBDCD1BF6A33CC6A1DC|YphC], [protein|DF1A043F3D9817D9479B8C707F4ACEEF2BF2862C|YsxC], [protein|959C0ED7B9DA50F0EADCF1565405B878D36DA206|YqeH], [protein|66A6EC4F6CB91EE741BB9D7646913FF3EE602BD7|ThdF], [protein|C0F5FAD6DF89DA9E0FFC8F888D7CED6AA42D9C73|YyaF], [protein|9F6B78C1932FB56F7F71430DB16BC862A312407B|YnbA]

    • [SW|Domains]

  • RNA-binding domain at the C-terminus [Pubmed|23325847]

    • Effectors of protein activity

  • GTPase activity requires binding of [gene|C5CAED1256F082E722EBA0A72647AA043B5681DB|L16]]] to the 50S subunit of the [SW|ribosome] [Pubmed|24335279]
  • binds ppGpp [Pubmed|26951678], ppGpp may inhibit activity [Pubmed|26951678]

    • Structure

  • [PDB|1PUJ]

    • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)

    • Expression and Regulation

    Operons

    genes
    [gene|54CD247926F7FABE4ACE85EB7021B62500B47260|rbgA]-[gene|D5275ECDC2A70FBAC2CA5980F04B272C3328FC90|rnhB]-[gene|0D4A4A1B2FD9D0698D0A65010A8ABC84DA7386A2|ylqG]-[gene|3F271D9FFE4F30D845319CD71662695AA592EC8F|ylqH]
    description
    [Pubmed|22383849]
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    Biological materials

    Mutant

  • MGNA-B141 (ylqF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1140 NBRP B. subtilis, Japan]

    • Labs working on this gene/protein

  • [SW|Naotake Ogasawara], Nara, Japan

    • References

    Reviews

  • 19575570,21885683

    • Original publications

  • 12427945,16390447,16997968,23325847,22267738,23700310,24335279,25330043,26951678